Arbovirus plaquing in a clonal line (PS Y-15) of porcine kidney.

Plaque formation by influenza viruses in a clonal line of porcine kidney cells.

MATSUDA, S. and YAOI, H. Plaque Formation by Influenza Viruses in a Clonal Line of Porcine Kidney Cells. Tohoku J. exp. Med., 1971, 103(2), 217-219 -The plaque formation by several strains of influenza virus was investigated in a clonal cell line of procine kidney (PS, clone Y-15). Among strains tested, PR8, NWS, and Lee formed well defined plaques and showed a linear dose response relationship...

Use of a clonal line of porcine kidney cell cultures for primary isolation and vaccine studies with adenoviruses.

The clonal line (Y15) of porcine kidney stable cells provided a recovery system for adenovirus T4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. Adenoviruses T7 from adults, and T1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. The latter were useful for adenovirus vaccine studies in that infect...

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene.

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for t...

Plaque assay of African Horse-Sickness Virus in African Green Monkey Kidney Cell Line

Genetic Adaptation of Porcine Circovirus Type 1 to Cultured Porcine Kidney Cells Revealed by Single-Molecule Long-Read Sequencing Technology

Porcine circovirus type 1 (PCV1) is a nonpathogenic circovirus, and a contaminant of the porcine kidney (PK-15) cell line. We present the complete and annotated genome sequence of strain Szeged of PCV1, determined by Pacific Biosciences RSII long-read sequencing platform.